There have been numerous scientific researches about Yunnan Baiyao including
"Yunnan Baiyao - where's the clinical evidence " by Lynelle Graham of University of Minnesota College of Veterinary Medicine.
Yunnan Baiyao is inexpensive, patent Chinese herb known for its positive hemostatic effects in the face of active hemorrage. A variety of traditional mechanisms for the action of yunnan baiyao have been proposed, including shortened bleeding time, shortened clotting times, reduced capillary permeability, peripheral vasoconstriction, an increased permeability of platelet membranes resulting in a release of platelet constituents, a calcium mediated effect. Some of these mechanisms and the contents of yunnan baiyao are defined by research, but many open questions remain regarding its use in companion animals. Preoperative use of yunnan baiyao may decrease the need and the cost of blood products. This could decrease the potential difficulties and the side effects of blood replacement and minimize the physiological stresses to the patient and the veterinary staff associated with blood loss.
Here is the list of some researches and studies about Yunnan Baiyao, a Chinese medicine for stopping bleeding :
The effect of Yunnan Baiyao on reduction of intra-operative bleeding of the patients
undergoing transurethral resection of prostate. Li NC, Pan BN, Wang HJ, Li HZ, Wei Q, Wang XH,
Cai SL, Gan WM, Na YQ, (2007) Zhonghua Yi Xue Za Zhi
Institute of Urology, Peking University First Hospital, Beijing 100034, China.
OBJECTIVE: To investigate the effect of Yunnan Baiyao on reduction of intra-operative bleeding of the patients undergoing transurethral resection of prostate (TURP). METHODS: A randomized placebo-control double-blind multi-center trial was conducted. 203 patients with benign prostate hyperplasia (BPH), aged 50 - 80, who were to undergo TURP were randomly divided into 2 groups: Yunnan Baiyao group, given 500 mg Yunnan Baiyao four times daily since the third day before the operation for 3 days, and placebo group, given empty capsules for 3 days. The amount of intra-operative bleeding, bleeding index and bleeding intensity were compared. The side effect of Yunnan Baiyao was also evaluated. RESULTS: The amount of intra-operative bleeding of the Yunnan Baiyao group was 147 ml +/- 144 ml, significantly less than that of the control group (182 ml +/- 157 ml, P < 0.05). The amount of intra-operative bleeding of the per protocol (PP) population in the Yunnan Baiyao group (n = 58) was 146 ml +/- 116 ml, significantly less than the PP population in the control group (n = 66) (220 ml +/- 207 ml, P = 0.0361). When the weight of the resected prostate tissue was more than 20 gram, the amount of intraoperative bleeding, bleeding index, and bleeding intensity of the ITT population in the Yunnan Baiyao group (n = 60) were all significantly lower than those of the ITT population in the control group (n = 68) (all P < 0.05); the bleeding intensity of the PP population in the Yunnan Baiyao group was 2.4 ml/min +/- 1.8 ml/min, significantly lower than that of the PP population in the control group (3.6 ml/min +/- 3.7 ml/min, P = 0.0072); the bleeding index of the PP population in the Yunnan Baiyao group was 4.7 ml/g +/- 4.6 ml/g, significantly lower than that of the PP population in the control group (7.1 ml/g +/- 8.3 ml/g, P = 0.025). No side effect was found in the 2 groups. CONCLUSION: Yunnan Baiyao is effective on reducing the amount of intraoperative bleeding of TURP without side effect.

Histological study on repairing experimental pulp chamber floor perforations with Yunnan Baiyao in the dog. Wang(1994), Zhongguo Zhong Xi Yi Jie He Za Zhi
College of Stomatology, Norman Bethune University of Medical Sciences, Changchun.
Yunnan Baiyao (YNBY), amalgam, hydroxylapatite and calcium hydroxide were used separately in repairing the experimental pulp chamber floor perforation (PCFP) of dog teeth. The animals were killed at the time of 1, 4, 8 and 12 weeks after experiment. The experimental teeth with periapical tissue were removed and observed their histological changes by optical microscope. The effects that different materials in repairing the tissue of PCFP were observed. Twenty teeth were taken from every dog, each group contained four teeth, the others were used for control group. Results: In initial period (1st, 4th week) inflammatory infiltration was present in PCFP of every group, but the group with YNBY was milder. Pyogenic foci were present in the hydroxylapatite and calcium hydroxide groups. In late stage of experiment (8th and 12th week) inflammatory reactions of each group were alleviated except control group. Epithelial metaplasia and cicatritation were found in the vicinity of perforated area. Dentin cementum and alveolar bone were absorbed in majority of experimental group. It was also observed in the group with YNBY. But in late stage similar cementum formation and alveolar bone neogenesis were found in the perforated area around the repairing materials. It revealed that hemostasis, Promoting Blood Circulation to Remove Stasis and antiphlogistic actions were advantageous to the inflammation, control and repair course of the PCFP. The article discussed that YNBY might promote the repairing of cementum and alveolar bone of perforated area.

Effects of Yunnan Baiyao on peri-operative bleeding of patients undergoing cervical
open-door laminoplasty: a multi-center randomized double-blind placebo-control trial. Pan SF, Sun Y,
Li F, Li GH, Jin DD, Zhang ZM, Luo ZJ, Ye ZX, Hu JH, Qiu GX, (2006), Zhonghua Yi Xue Za Zhi
Department of Orthopedics, Third Hospital of Peking University, Beijing 100083, China.
OBJECTIVE: To investigate the effects of Yunnan Baiyao on peri-operative bleeding of the patients undergoing cervical open-door laminoplasty. METHODS: 197 patients undergoing cervical open-door laminoplasty were randomly divided into 2 groups Yunnan Baiyao group (receiving Yunnan Baiyao 500 mg three times daily for 5 days) and placebo group. The amounts of intra-operative and post-operative bleeding were compared, and the side effect of Yunnan Baiyao was also analyzed. RESULTS: Statistical analysis showed that the amount of intra-operative bleeding of the Yunnan Baiyao was 350 ml +/- 190 ml, significantly lower than that of the placebo group (443 ml +/- 266 ml, P < 0.05). There were no statistical differences in the amount of postoperative bleeding and side effect rate between the two groups. CONCLUSION: Yunnan Baiyao is effective and safe in reducing the amount of intra-operative bleeding of cervical open-door laminoplasty.

Determination of panaxadiol and panaxatriol in radix notoginseng and Yunnan baiyao by capillary
supercritical fluid chromatography. Li YH, Li XL, Hong L, Liu JY, Zhang MY(1991) Yao Xue Xue Bao
School of Pharmacy, Second Military Medical University, Shanghai.
Capillary supercritical fluid chromatography (SFC) was developed for the determination of panaxadiol and panaxatriol in Radix notoginseng and Yunnan baiyao. 0.1g Radix notoginseng powder or 0.5g Yunnan baiyao was mixed with 10 ml 15% H2SO4 ethanol-water (1:1) solution, adding 1 mg cholesterol as internal standard. The mixture was refluxed for 4 h, then adding 15 ml 15% NaOH solution, refluxed for 0.5 h. The mixture was extracted 3 times with 10 ml portions of cyclohexane. The cyclohexane extracts were purified by partition column and concentrated by adsorption column and then analysed by SFC. The proposed method is sensitive, accurate, precise, simple and rapid; all the process can be done in 8 h.

Determination of diosgenin in Rhizoma Paridis by high performance liquid chromatography. Wei J, Dong X (1999)
Institute of Yunnan Baiyao Group Co. Ltd, Kunming 650032, China.
A method for the separation and determination of diosgenin in Rhizoma Paridis by reversed-phase high performance liquid chromatography was developed. The Rhizoma Paridis powder samples were extracted with methanol in a Soxhlet extractor. After extraction the solvent was evaporated and the extract was hydrolysed with 2 mol/L hydrochloric acid for 2 h in a boiling water bath. Then the diosgenin was extracted with petroleum ether (b.p. 60-90 degrees C). The operating conditions were Symmetry C8 column (5 microns, 3.9 mm x 150 mm) at 30 degrees C, mobile phase of V(acetonitrile):V(water) = 75:25 and UV detector at 203 nm. The linearity of the calibration curve was good in the range of 2.1-10.5 micrograms for diosgenin(r = 0.9994). The average recovery and RSD of diosgenin were 99.1% and 1.7% (n = 5) respectively. The method is accurate and reproducible and has been applied to the analysis of Rhizoma Paridis from different sources.

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